HistologyLesson 1 of 1 in the course Histological techniques

Histology: Tissue Classification, Preparation, Staining, and Microscopy

5 flashcards · 5 quiz questions · 5 match pairs · 5 fill-in sentences · English

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Summary

Histology is the study of biological tissues at the microscopic level. This lesson introduces the field, explains how tissues are classified, and explores how histology supports medicine and developed as a scientific discipline.

Histology, also called microscopic anatomy, microanatomy, or histoanatomy, studies the microscopic structure of biological tissues. It complements gross anatomy, which examines structures visible without a microscope.

All flashcards

Histology
The study of the microscopic structure of biological tissues; also called microscopic anatomy.
Histotechnology
The preparation of tissue specimens for microscopic examination.
Immunohistochemistry
A method that uses antibodies to visualize specific molecules in tissue.
Cryosectioning
Rapidly freezing tissue, cutting thin sections, and mounting them on slides.
Histological artifact
A feature introduced or altered during tissue collection or processing that can interfere with examination.

Quiz questions

  1. Which fixative is commonly used for light-microscopy tissue preparation?Answer: 10% neutral buffered formalin
  2. Before paraffin can infiltrate tissue, which processing sequence removes water and replaces the alcohol?Answer: Ethanol dehydration followed by clearing
  3. What distinguishes immunohistochemistry from a general tissue stain?Answer: It uses antibodies to visualize specific molecules in tissue.
  4. Which substances are commonly used to increase contrast in electron microscopy sections?Answer: Uranyl acetate and lead citrate
  5. Why can cryosectioning be useful during surgery?Answer: It allows rapid examination of tissue, such as tumor margins.

Based on the Wikipedia article Histology, licensed CC BY-SA 4.0. Adapted into a summary, flashcards and quizzes.

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